Inhibition of insulin/IGF-1 signaling suppresses lysosome damage and extends the shortened lifespan of scav-3(qx193). (A–E) Lifespan analyses in the indicated strains. More than 100 worms were quantified in each strain. Kaplan–Meier method followed by log-rank test was performed to compare all the other datasets with wild type (WT) or those that are linked by lines. *, P < 0.05; **, P < 0.0001; N.S., no significance. (F–N) Confocal fluorescent images of the hypodermis in the indicated strains expressing sfGFP::Gal3 at day 1 (F–H), day 3 (I–K), and day 6 (L–N) of adulthood. Animals were cultured and examined at 25°C, the nonpermissive temperature of the daf-2(e1370ts) mutation. (O) The number of sfGFP::Gal3-positive membrane-like structures was quantified in hypodermal cell 7 (hyp7) of the indicated strains as shown in F–N. Data are shown as mean ± SD. At least 15 worms were scored in each strain for each stage or condition. Two-way ANOVA with the Bonferroni posttest was performed to compare datasets that are linked by lines. *, P < 0.05; **, P < 0.001. (P–W) Confocal fluorescent images of the hypodermis in the indicated strains expressing Phsp-4GFP treated with DMSO (P–S) or tunicamycin (T–W). (X) The survival of worms after heat-shock treatment was quantified in the indicated strains. At least 100 worms were scored in each experiment, and three independent experiments were performed. Data are shown as mean ± SD. One-way ANOVA with Tukey’s posttest was performed to compare datasets that are linked by lines. **, P < 0.0001. Bars, 10 µm.
