The number of sfGFP::Gal3-positive damaged lysosomes in scav-3(qx193) is not affected by mutations in autophagy genes. (A–O) Confocal fluorescent images of the hypodermis in the indicated strains expressing sfGFP::Gal3 at L4 stage (A–F) and day 1 (G–L) and day 6 (M–O) of adulthood. (P and Q) The number of sfGFP::Gal3-positive membrane-like structures was quantified in hypodermal cell 7 (hyp7) of the indicated strains as shown in A–O. Data are shown as mean ± SD. At least 15 worms were scored in each strain at each stage. One-way ANOVA with Tukey’s posttest was performed to compare datasets that are linked by lines. **, P < 0.0001; N.S., no significance; WT, wild type. Bars, 10 µm.