GEF-H1 is required for MT depolymerization–induced neutrophil polarization and random migration. (A) GEF-H1^+/+ and GEF-H1^−/− neutrophils were treated with DMSO or nocodazole (Noc) for 30 min or fMLP for 5 min, and random migration was monitored for 10 min. Motion tracks from a representative experiment are shown. Distance from the origin is indicated on x and y axes in micrometers. Mean percentage of migrating cells (B) and the mean cell displacement (C) ± SEM are indicated and were determined from three independent experiments. More than 100 cells were tracked per experiment. Moving cells were defined as those that migrated a minimum distance of 15 µm.