Overexpression of LMP-1 and LMP-2 suppresses accumulation of damaged lysosomes in scav-3(lf). (A–H) Confocal fluorescent images of the hypodermis in the indicated strains expressing sfGFP::Gal3. Very few sfGFP::Gal3-positive membrane-like structures were seen in lmp-1, lmp-2, and lmp-2 lmp-1 worms (arrows). (J–O) Confocal fluorescent images of the hypodermis in scav-3(qx193) expressing sfGFP::Gal3 and LMP-2, wild-type (WT) LMP-1, glycosylation-defective LMP-1, wild-type SCAV-3, or glycosylation-defective SCAV-3. (S–T″) Confocal fluorescent images of the hypodermis in scav-3(qx193) expressing NUC-1::mCherry and SCAV-3(3N-1)::GFP or LMP-1(3N)::BFP. SCAV-3(3N-1) and LMP-1(3N) are localized to lysosomes (arrowheads). In panels I and P–R, the number of sfGFP::Gal3-positive membrane-like structures (I and P) or the percentage of rescue or suppression activity (Q and R) was quantified in hypodermal cell 7 (hyp7) of the indicated strains at day 1 of adulthood as shown in A–H and J–O. Data are shown as mean ± SD (I and P) or the distribution of rescue or suppression activity, with black lines representing the mean activity (Q and R). At least 15 animals were scored in each strain. One-way ANOVA with Tukey’s posttest was performed to compare all the other datasets with wild type or the nontransgenic control or to compare datasets that are linked by lines. *, P < 0.05; **, P < 0.0001; N.S., no significance. Bars, 10 µm.