Loss of scav-3 causes leakage of lysosome luminal hydrolases. (A–D) Time-lapse images of lysosomes in scav-3(qx193) expressing sfGFP::Gal3 and NUC-1::mCherry (A), GBA-3::mCherry (B), CPL-1::mCherry (C), or CPR-6::mCherry (D). 0 min represents the time point before initial appearance of sfGFP::Gal3 around the lysosome (arrows). (E–F″) Confocal fluorescent images of the hypodermis in wild-type (WT; E–E″) and cup-5(bp510) (F–F″) adults expressing sfGFP::Gal3 and NUC-1::mCherry. Gal3 was diffuse in the cytosol in wild-type and in cup-5 mutants that contained enlarged lysosomes (arrowheads). Bars, 5 µm.