Figure 4.
Loss of scav-3 does not affect targeting of lysosomal hydrolases. Merged confocal fluorescent images of the hypodermis in wild-type (WT; A, C, E, G, I, and K) and scav-3(qx193) (B, D, F, H, J, and L) larvae coexpressing LAAT-1::GFP and GBA-3::mCherry (A and B), GBA-1::mCherry (C and D), CPL-1::mCherry (E and F), CPR-6::mCherry (G and H), Y105E8B.9::mCherry (I and J), or ASM-1::mCherry (K and L). The hydrolases were seen in LAAT-1::GFP-positive globular (arrowheads) and tubular (arrows) lysosomes in wild type and enlarged vesicular lysosomes (arrowheads) in scav-3(qx193) mutants. Bars, 10 µm.