SCAV-3 is widely distributed and localizes to lysosomes. (A–F′) Differential interference contrast (DIC) and confocal fluorescent images of wild type expressing SCAV-3::GFP driven by the scav-3 promoter. (G–H″) Confocal fluorescent images of the hypodermis in wild type expressing SCAV-3::GFP and LAAT-1::mCherry (G–G″) or SCAV-3::GFP and NUC-1::mCherry (H–H″). SCAV-3 colocalizes with LAAT-1 and NUC-1 to both vesicular (arrowheads) and tubular (arrows) lysosomes. (I and J) Fluorescent images of wild-type animals expressing SCAV-3::sfGFP (I) or SCAV-3(AAA)::sfGFP (J) in the pharynx. SCAV-3(AAA)::sfGFP localizes to plasma membranes (arrows). (K–S) Confocal fluorescent images of wild type expressing LMP-1::sfGFP (K–R) or LMP-2::sfGFP (S). (T) The mean total fluorescence intensity of LMP-1::sfGFP in different cell types was measured, and the normalized florescence intensity is shown. At least 15 animals were scored in each cell type. Data are shown as mean ± SD. One-way ANOVA with Tukey’s posttest was performed to compare all the other datasets with hypodermis. *, P < 0.05; **, P < 0.0001. Bars, 10 µm.