scav-3 mutants accumulate abnormal lysosomes. (A–F″) Confocal fluorescent images of embryos (A–B″) and the hypodermis of larvae (C–D″) and adults (E–F″) in wild type (WT; A–A″, C–C″, and E–E″) and scav-3(qx193) (B–B″, D–D″, and F–F″) expressing LAAT-1::GFP and NUC-1::mCherry. Arrowheads, globular lysosomes; white arrows, tubular lysosomal structures; blue arrows, abnormal tubule-like structures in scav-3(qx193). (G–I) Lysosome volumes in fourfold embryos (4F) or L1 larvae (L1) were quantified. At least 10 animals were scored in each strain at each stage. Data are shown as mean ± SD in I. Two-way ANOVA with the Bonferroni posttest was performed to compare mutant datasets with wild type. **, P < 0.001. (J) Time-lapse images of lysosomes in the hypodermis of L1 larvae of wild type and scav-3(qx193) expressing LAAT-1::GFP, with time point 0 s in green and 20 s in red. The overlay (merge) shows lysosome movement over time. White arrowheads and arrows, dynamic changes in globular and tubular lysosomes in wild type; blue arrowheads, static vesicular lysosomes in scav-3(qx193). Pearson’s correlation coefficient was determined and is shown in the right panel. Data are shown as mean ± SD. At least 10 movies were analyzed in each strain. Data were compared with the unpaired t test. **, P < 0.0001. Bars, 10 µm.