Figure 1. The duration of mitosis is modulated by nutrients. (A) Wild-type cells growing in YPD (rich carbon) or YPG/E (poor carbon) were arrested in G1 phase by addition of mating pheromone. The cells were released from arrest, and levels of mitotic cyclin Clb2 were assayed by Western blot. (B and C) Cells growing in YPD were released from G1 arrest. At 90 min, the culture was split, and one half was washed into YPD and the other half into YPG/E. Levels of mitotic cyclin Clb2 were assayed by Western blot (B), and cells with short metaphase spindles were assayed by immunofluorescence (C). (D and E) Cells growing in YPD were released from G1 arrest. At 105 min, the culture was split, and one half was washed into YPD and the other half into YPG/E. Levels of mitotic cyclin Clb2 were assayed by Western blot (D), and long anaphase spindles were assayed by immunofluorescence (E). Numbers on the left side of Western blots indicate molecular mass in kilodaltons.
Figure 1.

The duration of mitosis is modulated by nutrients. (A) Wild-type cells growing in YPD (rich carbon) or YPG/E (poor carbon) were arrested in G1 phase by addition of mating pheromone. The cells were released from arrest, and levels of mitotic cyclin Clb2 were assayed by Western blot. (B and C) Cells growing in YPD were released from G1 arrest. At 90 min, the culture was split, and one half was washed into YPD and the other half into YPG/E. Levels of mitotic cyclin Clb2 were assayed by Western blot (B), and cells with short metaphase spindles were assayed by immunofluorescence (C). (D and E) Cells growing in YPD were released from G1 arrest. At 105 min, the culture was split, and one half was washed into YPD and the other half into YPG/E. Levels of mitotic cyclin Clb2 were assayed by Western blot (D), and long anaphase spindles were assayed by immunofluorescence (E). Numbers on the left side of Western blots indicate molecular mass in kilodaltons.

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