ROS-induced actin and tubulin glutathionylation controls cell cytoskeleton dynamics and NET formation. Physiological activation of neutrophils leads to moderate ROS production and cell cytoskeleton glutathionylation. ROS-induced actin and tubulin glutathionylation is tightly regulated by Grx1. Disruption of Grx1 or deficient ROS production (in CGD patients) unbalances cell cytoskeleton rearrangements and leads to a dysfunctional innate immune response in neutrophils. Confocal microscopy images are back projections of 30 z-stacks, and a visualization of the tubulin (purple) isosurface rendering was applied for better visualization by using Imaris software. Bars, 10 µm.