Figure 4.
Figure 4. Targeted secretion of lytic granules promotes more killing of the target cells. Live-cell confocal microscopy of YTS-CD16 cells conjugated with S2-IgG (A) or S2-IC1-IgG (B) cells. NK cells were mixed with the target cells immediately before the imaging process. Cell mixtures were imaged every 5 min for 2 h. Time zero represents the start of imaging. Yellow, S2-IgG or S2-IC1-IgG cells; red, LysoTracker red (lytic granules); blue, SYTOX blue viability dye. Quantitative analyses of viable cells are shown as a feature of the differential killing efficiency. Arrowheads indicate uptake of SYTOX blue viability dye by the target cells. (C) Live granule tracking in YTS-CD16 cells conjugated with S2-IgG and S2-IC1-IgG cells, respectively. Each point indicates one independent experiment using YTS-CD16 (D) and eNK (E) cells (n > 300 cells/group). Error bars show ± SD. *, P < 0.05; **, P < 0.01; ****, P < 0.0001.

Targeted secretion of lytic granules promotes more killing of the target cells. Live-cell confocal microscopy of YTS-CD16 cells conjugated with S2-IgG (A) or S2-IC1-IgG (B) cells. NK cells were mixed with the target cells immediately before the imaging process. Cell mixtures were imaged every 5 min for 2 h. Time zero represents the start of imaging. Yellow, S2-IgG or S2-IC1-IgG cells; red, LysoTracker red (lytic granules); blue, SYTOX blue viability dye. Quantitative analyses of viable cells are shown as a feature of the differential killing efficiency. Arrowheads indicate uptake of SYTOX blue viability dye by the target cells. (C) Live granule tracking in YTS-CD16 cells conjugated with S2-IgG and S2-IC1-IgG cells, respectively. Each point indicates one independent experiment using YTS-CD16 (D) and eNK (E) cells (n > 300 cells/group). Error bars show ± SD. *, P < 0.05; **, P < 0.01; ****, P < 0.0001.

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