No single factor is a good predictor of NPC permeability. (A) Effect of select features on pore basal permeability (p/pWT) relative to the WT strain. Shown are the four features with the highest absolute correlation. Plots for all 33 features are shown in Fig. S5. (B) Predicted basal permeability (p/pWT) of combinatorial deletion strains (±SEM) relative to the WT strain according to the LASSO statistical regression model versus the experimental measurements for each strain. (C) Aggregate contribution of features related to either the ratio of net charge to hydropathy or position to the LASSO regression model. (D) p/pWT of individual nups relative to the WT strain according to the regression model. The nups are positioned based on their proximity to the cytoplasm (z axis) and their distance from the NPC radial axis, as observed in immuno-EM maps (Alber et al., 2007b). The prediction for Nup159 should be assigned lower confidence because of the unusual negative charge and architecture of Nup159.
