Size-dependent permeability in four FG deletion strains. (A) An example of NPC permeability assays from representative single cells expressing GFP-2PrA, from FG deletion and control strains. Bars, 1 µm. The permeability of Δnup170 was measured as positive control for a previously published leaky strain (Shulga et al., 2000). (B) Strains in which mean transport times () were calculated for all six different-sized substrates are plotted ± 95% confidence intervals as a function of the reporter molecular masses in log-log scale, these being 29 (GFP), 34 (GFP-1PrA), 41 (GFP-2PrA), 47 (GFP-3PrA), 54 (GFP-4PrA), and 67 kD (GFP-6PrA). As GFP-xPrG data were only collected for WT cells, only GFP-xPrA data are included in this figure, for more direct comparisons. The solid line in each subplot indicates the fit to a power function. For the mutant strains, the WT fit is superimposed as a red line. The functional form of each power function is indicated along with the 95% confidence intervals for the power exponent.
