Permeability of mutant strains. (A) Combinatorially deleted strains used in this study; deleted nups in each strain are indicated by white circles. Bottom left illustrates that each diagram represents the right half of a cross section of a diagrammatic NPC, where R is the radial distance from the pore radial axis, and the z axis denotes vertical proximity to the cytoplasm (positive values) or the nucleus (negative values). These approximate FG nup positions are drawn according to the localization map for the FG nup C termini used in Table S2 of reference (Alber et al., 2007a). FG nup diameters are estimated for visualization purposes using Eq. 6 in reference (Marsh and Forman-Kay, 2010). (B) Measured NPC permeability coefficients (p) in different strains for GFP-2PrA (molecular mass 40.7 kD) and GFP-4PrA (53.6 kD). Each black dot is from one single-cell FRAP measurement. Red dots are the mean for the GFP-2PrA or GFP-4PrA substrate in that strain. Δnup170 was measured as positive control for a previously published “leaky strain” (Shulga et al., 2000). (C) Permeability of GFP-2PrA versus GFP-4PrA in the different strains relative to the permeability of the WT strain (±SEM). Strains lacking Nup100 or Nup116 GLFG domains are marked red, the nup49ΔFGnup57ΔFG strain green, WT black, Δnup170 gray, and all others blue.
