CD16 engagement induces conjugate formation and degranulation in human NK cells. For conjugation assay, numbers indicate the percentage of YTS-CD16 cells (A), NK92-CD16 cells (B), and previously cryopreserved eNK cells (C) in conjugates. Data represent results from three independent experiments using NK cell lines or eNK cells from three healthy donors. Error bars indicate + SD (A−C). For degranulation assay, combined results from seven experiments for for YTS-CD16 cells (D) and three experiments for NK92-CD16 cells (E) showed significantly higher degranulation levels of NK cells co-cultured with S2-IC1-IgG cells compared with S2-IgG cells. (F) Data from three healthy donors showed comparable degrees of degranulation by eNK cells coincubated with S2-IgG and S2-IC1-IgG cells. Error bars show ± SD (D−F). **, P < 0.01; ns, not significant.