Figure 4. The VPS35–N-Ras interaction is dependent on prenylation, but not palmitoylation or GTP binding. (A–D) HEK293 cells were transiently transfected with plasmids directing expression of the indicated proteins and analyzed by immunoprecipitation and immunoblot as in Fig. 3. Before lysis, cells were treated with farnesyltransferase inhibitor (FTI) and/or geranylgeranyltransferase inhibitor (GGTI; A) or 2-bromopalmitate (B). (D) Lysates were treated with GTP-γS or GDP before immunoprecipitation (IP). Data shown are representative of at least three independent experiments.
Figure 4.

The VPS35–N-Ras interaction is dependent on prenylation, but not palmitoylation or GTP binding. (A–D) HEK293 cells were transiently transfected with plasmids directing expression of the indicated proteins and analyzed by immunoprecipitation and immunoblot as in Fig. 3. Before lysis, cells were treated with farnesyltransferase inhibitor (FTI) and/or geranylgeranyltransferase inhibitor (GGTI; A) or 2-bromopalmitate (B). (D) Lysates were treated with GTP-γS or GDP before immunoprecipitation (IP). Data shown are representative of at least three independent experiments.

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