![Figure 3. VPS35 interacts with N-Ras in a prenyl-dependent fashion. (A and B) HEK293 cells were transiently transfected with VPS35 and/or N-Ras (wild type or C186S; A); N-Ras and either VPS35, VPS26A, or VPS29 (B); or all three. Whole-cell lysates (input) were immunoprecipitated with an anti-FLAG antibody (FLAG immunoprecipitation [IP]), and samples were analyzed by immunoblot. Data shown are representative of four independent experiments. (C) The cytosol (S350) of MDCK cells was subjected to Superdex 75 chromatography, and the indicated endogenous protein levels were analyzed by immunoblot. Data shown are representative of at least three independent experiments.](https://cdn.rupress.org/rup/content_public/journal/jcb/214/4/10.1083_jcb.201604061/5/jcb_201604061_fig3.jpeg?Expires=1767669769&Signature=3Z9Xm5KvpJ64eHGguxXn4J1dDBjhw8U25zdxo1yotI7aYpuinMXK6ROaQAbcmZADeoQwH1e9UiQzcXbCZAinuMzTVGg73W8K0iq5LHF533qmjvw-1n0bXCV~trjMg3JyUI~mUSUdtJdo02UfJmU228SVHaLF7IULt00ktdpT8pU7FlYFbdbRRr2WQbb3IZyN78b2QsSplCDFsJgU0y7ykdz0Bxu16m6~m6Y72e5EX7rj2ly3pSaxj4QDadm6e1hWunqk0pHwWnskXt-aq-PIEh~B491XqtPSKTm1D~mXpH0beHaMHEFAeg~VUWzwsaSy9XGic9uOo3cMru8OZ3kEpw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Figure 3.
VPS35 interacts with N-Ras in a prenyl-dependent fashion. (A and B) HEK293 cells were transiently transfected with VPS35 and/or N-Ras (wild type or C186S; A); N-Ras and either VPS35, VPS26A, or VPS29 (B); or all three. Whole-cell lysates (input) were immunoprecipitated with an anti-FLAG antibody (FLAG immunoprecipitation [IP]), and samples were analyzed by immunoblot. Data shown are representative of four independent experiments. (C) The cytosol (S350) of MDCK cells was subjected to Superdex 75 chromatography, and the indicated endogenous protein levels were analyzed by immunoblot. Data shown are representative of at least three independent experiments.
