Blockade of nuclear calcium signaling induces an anergy-like state in activated human T cells. QRT-PCR analysis of IL-2 expression in human T cells transfected with expression vectors for CaMBP4.GFP (CaMBP4) or GFP. Cells were stimulated as indicated with CD3 antibodies alone or with a combination of CD3 and CD28 antibodies (for 2 h) with or without overnight pretreatment with CD3 antibodies (n = 3). The results are expressed relative to the IL-2 mRNA levels of unstimulated GFP-expressing T cells. Statistically significant differences are indicated with an asterisk (*, P < 0.05). Error bars represent SEM.