Regulation of chromosome alignment by ASB7 and DDA3. (A) Maximum projections from deconvolved z stacks of representative ASB7, DDA3, and/or Kif2a knockdown HeLa cells or HeLa cells stably overexpressing 3×HA-DDA3, as well as control cells, immunostained for β- or γ-tubulin. DNA was stained with Hoechst 33258. (B) Proportion of mitotic cells with unaligned chromosomes. More than 150 mitotic HeLa cells were counted, and percentages of cells with unaligned chromosomes are shown. (C) Proportion of mitotic cells with unaligned chromosomes. More than 150 mitotic HeLa cells were counted, and percentages of cells with unaligned chromosomes are shown. *, P < 0.05; **, P < 0.01. Data represent means ± SD. (D) Maximum projections from deconvolved z stacks of representative ASB7 knockdown or ASB7-complemented HeLa cells, immunostained for β- or γ-tubulin. DNA was stained with Hoechst 33258. Bars, 5 µm.