The localization of B2AR to ASRT or bulk recycling domains does not influence global cAMP levels. (A) Example images of Epac and B2AR. 1–2 min after iso, the CFP/FRET ratio increases as cAMP is produced and FRET decreases. Bar, 10 µm. (B) CFP/FRET ratio across multiple B2AR cells. Error bars are SEM across cells. n = 10 cells. (C) The total cAMP produced at 20 min increased significantly for both B2AR and SS>AA. Data are Tukey box plots from 13 (B2AR) and 10 (SS>AA) cells.