Figure 3. A phosphomimetic B2AR mutant is restricted to ASRT domains independent of PKA and activates Gαs in ASRT domains. (A) Example images of B2AR and SS>DD ASRT (arrowheads) and non-ASRT (arrows) tubules after iso and before and after KT. Bar, 2 µm. (B) Percent ASRT tubules (marked by coronin) and non-ASRT tubules for B2AR and SS>DD with and without KT. KT does not cause SS>DD to enter non-ASRT tubules. n = 43 (B2AR − KT), 26 (B2AR + KT), 64 (SS>DD − KT), and 36 (SS>DD + KT) cells. n.s., not significant. (C) Example images of FLAG-B2AR and SS>DD with cortactin and Nb37 after iso. Nb37 is recruited to SS>DD ASRT tubules (arrowheads). Bar, 2 µm. (D) Percent tubules per cell that contain cortactin and Nb37, cortactin or Nb37 only, or neither marker. n = 42 (B2AR) and 47 (SS>DD) cells. Error bars in all graphs are SEM across cells.
Figure 3.

A phosphomimetic B2AR mutant is restricted to ASRT domains independent of PKA and activates Gαs in ASRT domains. (A) Example images of B2AR and SS>DD ASRT (arrowheads) and non-ASRT (arrows) tubules after iso and before and after KT. Bar, 2 µm. (B) Percent ASRT tubules (marked by coronin) and non-ASRT tubules for B2AR and SS>DD with and without KT. KT does not cause SS>DD to enter non-ASRT tubules. n = 43 (B2AR − KT), 26 (B2AR + KT), 64 (SS>DD − KT), and 36 (SS>DD + KT) cells. n.s., not significant. (C) Example images of FLAG-B2AR and SS>DD with cortactin and Nb37 after iso. Nb37 is recruited to SS>DD ASRT tubules (arrowheads). Bar, 2 µm. (D) Percent tubules per cell that contain cortactin and Nb37, cortactin or Nb37 only, or neither marker. n = 42 (B2AR) and 47 (SS>DD) cells. Error bars in all graphs are SEM across cells.

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