Figure 2. B2AR activates Gαs exclusively in ASRT endosomal microdomains. (A) Example images of B2AR, an ASRT marker; cortactin; and Nb80. Nb80 localizes to endosomes and tubules (arrowheads). Bars, 5 µm. (B) Example images of B2AR and SS>AA with cortactin and Nb80. Nb80 localizes to ASRT (arrowheads) and non-ASRT SS>AA tubules (arrows). Bars, 2 µm. (C) Percent tubules per cell with cortactin and Nb80, cortactin or Nb80 only, or neither. n = 15 (B2AR) and 21 (SS>AA) cells. (D) Example images of B2AR, cortactin, and Nb37. Nb37 localizes exclusively to the base of B2AR tubules (arrowheads) with cortactin (ASRT). Bars, 5 µm. (E) Example images of B2AR and SS>AA with cortactin and Nb37. Nb37 is not recruited to SS>AA constitutive tubules (arrows). Arrowheads show B2AR in ASRT domains. Bars, 2 µm. (F) Quantitation of the percentage of total tubules per cell that contain cortactin and Nb37, cortactin or Nb37 only, or neither. n = 42 (B2AR) and 43 (SS>AA) cells.
Figure 2.

B2AR activates Gαs exclusively in ASRT endosomal microdomains. (A) Example images of B2AR, an ASRT marker; cortactin; and Nb80. Nb80 localizes to endosomes and tubules (arrowheads). Bars, 5 µm. (B) Example images of B2AR and SS>AA with cortactin and Nb80. Nb80 localizes to ASRT (arrowheads) and non-ASRT SS>AA tubules (arrows). Bars, 2 µm. (C) Percent tubules per cell with cortactin and Nb80, cortactin or Nb80 only, or neither. n = 15 (B2AR) and 21 (SS>AA) cells. (D) Example images of B2AR, cortactin, and Nb37. Nb37 localizes exclusively to the base of B2AR tubules (arrowheads) with cortactin (ASRT). Bars, 5 µm. (E) Example images of B2AR and SS>AA with cortactin and Nb37. Nb37 is not recruited to SS>AA constitutive tubules (arrows). Arrowheads show B2AR in ASRT domains. Bars, 2 µm. (F) Quantitation of the percentage of total tubules per cell that contain cortactin and Nb37, cortactin or Nb37 only, or neither. n = 42 (B2AR) and 43 (SS>AA) cells.

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