Kon is functionally related to Notch. (a) kon knockdown in glia did not affect Notch signaling, visualized with the Su(H)lacZ reporter. Overexpression of kon in glia caused a reduction in the number of abdominal anti-βGal⁺ cells in larvae. Student’s t test: **, P < 0.01. (b) kon knockdown in all glia enhanced the increase in glial cell number caused by the overexpression of activated Notch^ICD. Student’s t test: *, P < 0.05. (c) qRT-PCR revealing kon expression: kon expression decreased in Notch^ts mutant male hemizygous larvae (Student’s t test: ***, P < 0.001) and increased upon the overexpression of activated Notch^ICD in glia (one-way ANOVA, post hoc Dunnett comparison to control: **, P < 0.01); overexpression of activated Notch^ICD in glia in a pros^S044116 mutant background increased kon expression; Student’s t test: **, P < 0.05. ns, not significant. (d) Diagram illustrating the functional genetic relationships between kon, Notch, and pros in development. (a and b) Sample types are larval VNCs; (a) n = 4, 6; (b) n = 13, 13. (c) Sample types are dissected CNS; n = 20 CNS per genotype per replicate, three replicates per genotype. For further details, see Table S1; >, GAL4/UAS. Bars, 50 µm.