Ror2^Y324C downstream targets overlap with cell cycle–associated genes. (A) Schematic of experimental design. Single embryos were collected at E9.5, and Oct4-ΔPE-GFP⁺ PGCs were isolated by FACS. RNA was extracted from WT (Ror2^+/+) and Ror2^Y324C PGCs, converted to cDNA, amplified, and hybridized to the GeneChip Mouse Gene 1.0 ST Array (Affymetrix). Age and number of PGCs collected per embryo are shown in the table. (B) Microarray data from Ror2^Y324C PGCs compared with WT shown as log fold change versus log p-values for all annotated microarray probes (dChip). Each dot represents a single probe. Purple dots show genes with a P < 0.05 and a fold change >1.2 (not log scale). Green dots show significant genes that overlap with β-catenin ChIP in mouse intestinal crypts (Schuijers et al., 2015). (C) Functional analysis of >1.2-fold microarray hits by Ingenuity Pathway Analysis find cell cycle–associated genes to be significantly misregulated. Number of genes associated with each Gene Ontology category is shown on the right. Exemplary genes of interest for cell cycle and cell growth and proliferation categories are listed. (D) GSEA comparison of the Ror2^Y324C microarray data with a curated list of cell cycle genes from the QIAGEN Cell Cycle PCR Array suggests an enrichment for Ror2^Y324C-associated genes. P-value, enrichment score (ES), and normalized enrichment score (NES) are specified. Heatmap shows normalized microarray intensity values for the 15 most and 15 least enriched cell cycle–associated genes in Ror2^Y324C PGCs as identified by the GSEA. For each gene, dark blue color indicates the lowest probe intensity value (zeroth percentile), dark red indicates the highest probe intensity value (100th percentile), and white indicates the middle 50th percentile of intensity values.