Figure 5.
Figure 5. The role of Jak1 in stabilizing the ternary complex. (a) Receptor dimerization by IFNα2 (blue) and IFNα2-YNS (red) for full-length (fl) IFNAR2, IFNAR2 truncated after the Jak1 binding site (Δ346), and after the transmembrane domain (Δ265). (b and c) Binding of 2 nM DY647IFNα2-M148A (b) and DY647IFNα2-dn (c) to cell lines deficient in Jak1 (U4C) and IFNAR2 (U5A). For comparison, binding to the parental cell line (2fTGH) and to U4C complemented with Jak1 is shown. Box plots indicate the data distribution of the second and third quartile (box), median (line), mean (closed squares), and whiskers (1.5× interquartile range).

The role of Jak1 in stabilizing the ternary complex. (a) Receptor dimerization by IFNα2 (blue) and IFNα2-YNS (red) for full-length (fl) IFNAR2, IFNAR2 truncated after the Jak1 binding site (Δ346), and after the transmembrane domain (Δ265). (b and c) Binding of 2 nM DY647IFNα2-M148A (b) and DY647IFNα2-dn (c) to cell lines deficient in Jak1 (U4C) and IFNAR2 (U5A). For comparison, binding to the parental cell line (2fTGH) and to U4C complemented with Jak1 is shown. Box plots indicate the data distribution of the second and third quartile (box), median (line), mean (closed squares), and whiskers (1.5× interquartile range).

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