Figure 1. RNase sensitivity screening of NBs. (A) Brief procedure of the screening. Venus-tagged human FLJ cDNA clones were transfected into HeLa cells. cDNA clones whose products localized to certain nuclear foci were selected. Subsequently, the RNase sensitivity of the nuclear foci labeled by Venus was investigated. To this end, the cells were permeabilized with 2% Tween-20, followed by treatment with an RNase mixture. (B) Control experiment (Pyronin Y) and examples of RNase-resistant NBs (Cajal bodies marked by COIL-Venus) and RNase-sensitive NBs (paraspeckles marked by SFPQ-Venus). (C) Demonstration of the RNase-sensitive localization of four Venus-tagged proteins in nuclear foci selected from Table 1. Venus-C1orf108 is an example of RNase-resistant localization in nuclear foci. Arrowheads indicate nuclear foci. Bars, 10 µm.
Figure 1.

RNase sensitivity screening of NBs. (A) Brief procedure of the screening. Venus-tagged human FLJ cDNA clones were transfected into HeLa cells. cDNA clones whose products localized to certain nuclear foci were selected. Subsequently, the RNase sensitivity of the nuclear foci labeled by Venus was investigated. To this end, the cells were permeabilized with 2% Tween-20, followed by treatment with an RNase mixture. (B) Control experiment (Pyronin Y) and examples of RNase-resistant NBs (Cajal bodies marked by COIL-Venus) and RNase-sensitive NBs (paraspeckles marked by SFPQ-Venus). (C) Demonstration of the RNase-sensitive localization of four Venus-tagged proteins in nuclear foci selected from Table 1. Venus-C1orf108 is an example of RNase-resistant localization in nuclear foci. Arrowheads indicate nuclear foci. Bars, 10 µm.

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