Inhibition of AS Plk4 leads to centrosome loss and growth arrest. (A) Principle of AS Plk4 and its inhibition by 3MB-PP1. (B) Schematic of sgRNA and repair template oligo used to knock in the AS mutation at endogenous Plk4 genomic loci. sgRNA sequence is highlighted in blue, and edited nucleotides are displayed in red. (C) Centriole number distribution in interphase Plk4^AS cells at time points after addition of 3MB-PP1. Data are means ± SEM of three independent experiments (n = 3) with >80 cells per experiment. (D) Fold increase in cell number after 3MB-PP1 addition. Data are means ± SEM (n = 2, performed in triplicate). WT, wild type.