Figure 3.
Figure 3. Postnatal CMs with telomere shortening activate the DDR and form anaphase bridges. (A) Detail of telomere (Tel) Q-FISH and γH2AX/TnI immunofluorescence. Arrowheads indicate foci of the DNA-damage marker protein γH2AX at telomeres in a CM. Bars, 10 µm. (B) Quantification of the proportion of CMs with colocalized γH2AX and telomeres. n indicates the number of animals analyzed. (C) Confocal (left) and superresolution (right) images of DNA bridges in a dividing P8 CM. Arrowheads indicate a DNA bridge. Bars, 10 µm. (D) Proportion of anaphases-telophases with DNA bridges in CMs at P1 and P8. n indicates the number of animals analyzed per group. (E) P8 CM with a micronucleus. Bar, 25 µm. The arrowhead indicates the micronucleus. (F) Quantification of the proportion of CMs with a micronucleus at P1 and P8. n indicates the number of animals analyzed. Data are mean ± SEM. *, P < 0.05; **, P < 0.01.

Postnatal CMs with telomere shortening activate the DDR and form anaphase bridges. (A) Detail of telomere (Tel) Q-FISH and γH2AX/TnI immunofluorescence. Arrowheads indicate foci of the DNA-damage marker protein γH2AX at telomeres in a CM. Bars, 10 µm. (B) Quantification of the proportion of CMs with colocalized γH2AX and telomeres. n indicates the number of animals analyzed. (C) Confocal (left) and superresolution (right) images of DNA bridges in a dividing P8 CM. Arrowheads indicate a DNA bridge. Bars, 10 µm. (D) Proportion of anaphases-telophases with DNA bridges in CMs at P1 and P8. n indicates the number of animals analyzed per group. (E) P8 CM with a micronucleus. Bar, 25 µm. The arrowhead indicates the micronucleus. (F) Quantification of the proportion of CMs with a micronucleus at P1 and P8. n indicates the number of animals analyzed. Data are mean ± SEM. *, P < 0.05; **, P < 0.01.

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