Figure 2.
Figure 2. Proliferative CMs possess long telomeres. (A) Detail of a maximum-intensity projection confocal image of telomere Q-FISH and TnT/pH3 immunofluorescence. Proliferative (pH3+) and nonproliferative (pH3−) CMs were manually selected using the pH3 and TnT immunofluorescence images. The white arrowhead indicates a pH3− CM; the yellow arrowhead indicates a pH3+ CM. Bar, 25 µm. (B) Mean telomere length in pH3+ and pH3− CMs. (C) Telomere-length distribution and percentage of relatively short (<4,000 auf) and long telomeres (>4,000 auf) in proliferative and nonproliferative CMs. Gray lines at 4,000 auf facilitate comparisons of telomere size distribution between conditions. Red lines indicate the mean telomere length. n indicates the number of CMs analyzed per group. The number of animals is shown in brackets. Data are mean ± SEM. *, P < 0.05; ***, P < 0.001; NS, nonsignificant.

Proliferative CMs possess long telomeres. (A) Detail of a maximum-intensity projection confocal image of telomere Q-FISH and TnT/pH3 immunofluorescence. Proliferative (pH3+) and nonproliferative (pH3−) CMs were manually selected using the pH3 and TnT immunofluorescence images. The white arrowhead indicates a pH3− CM; the yellow arrowhead indicates a pH3+ CM. Bar, 25 µm. (B) Mean telomere length in pH3+ and pH3− CMs. (C) Telomere-length distribution and percentage of relatively short (<4,000 auf) and long telomeres (>4,000 auf) in proliferative and nonproliferative CMs. Gray lines at 4,000 auf facilitate comparisons of telomere size distribution between conditions. Red lines indicate the mean telomere length. n indicates the number of CMs analyzed per group. The number of animals is shown in brackets. Data are mean ± SEM. *, P < 0.05; ***, P < 0.001; NS, nonsignificant.

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