Specific paxillin and Hic-5 interactors. (A) SDS-PAGE (10% gel) and silver staining analysis of paxillin and Hic-5 binding partners copurified with either pax-WT-Flag or Hic-5-WT-Flag. The control condition corresponds to cell lysates of paxillin- and Hic5-deficient cells (pax^−/− and hic^−/− cells) with nonspecific IgG but with the same isotype as anti-Flag M2. (B) Cytoscape visualization of the specific and shared interactors between paxillin and Hic-5 (dark blue). In these cellular and purification conditions, paxillin interacts with numerous acto-adhesive regulators and the newly contractile regulator JAK1. Hic-5 interacts with less acto-adhesive regulators but can bind specifically to exocyst regulator IQGAP1.