Ndel1 negatively regulates primary cilia assembly in the kidney of newborn mice. (A) Total protein was extracted from brains or kidneys of NDEL1^WT/WT or NDEL1^cko/cko mice at 3 mo of age and subjected to immunoblotting. Relative amounts of Ndel1 were quantified using densitometry, normalized to the content of actin. (B) A male NDEL1^WT/WT (left) or NDEL1^cko/cko (right) mouse at 3 mo of age. (C) Mean body weight of NDEL1^WT/WT or NDEL1^cko/cko mice at 4 mo of age (n = 5 mice each). (D) hematoxylin and eosin staining of a kidney from a mouse. A red, blue, or green arrowhead indicates proximal or distal tubule or collecting duct (C.D.), respectively. (E) Kidney from the newborn NDEL1^WT/WT or NDEL1^cko/cko mice was stained with anti–acetylated tubulin (red), anti-Arl13b (green), and DAPI (DNA; blue). White dashed line indicates the border of each renal tubule/duct. (F and G) The percentage of ciliated cells (F) and mean length of primary cilia (G) in kidney of newborn mice are shown in the graphs. For calculation, we used >50 cells from n ≥ 5 (F) or 20 (G) mice per each category. (H) Kidney sections from the indicated newborn mice were stained with anti–acetylated tubulin (red) anti-Ki-67 (green) and DAPI (blue). (I) We quantified the percentage of Ki-67–positive cells, using >800 cells from three mice. Bars: (B) 1 cm; (E) 10 µm; (D) 20 µm; (H) 25 µm.