The microstructure of mother centriole/basal body in cells expressing Ndel1-Myc or MBP-trichoplein-Flag under cultivation without serum. (A and B) Each RPE1 cell line allowing inducible expression of Ndel1-Myc or MBP-trichoplein-Flag was cultured in the serum-free medium with 30 or 300 ng/ml Dox, respectively. 24 h after incubation, treated cells were subjected to TEM (A) or immunostaining (B). Micrographs show distinct mother centrioles and basal bodies (single sections). CB, ciliary bud; CV, ciliary vesicle; DA, distal appendage; SDA, subdistal appendage. Bars: (A) 200 nm; (B, main) 5 µm; (B, insets) 1 µm. (C) The cartoon shows our proposed model. P, phosphate; Ub, ubiquitin; Tricho, trichoplein; AurA, Aurora A.