Figure 7. Primary cilia assembly by Ndel1 depletion is reverted by trichoplein overproduction or KCTD17 coknockdown. (A) Tet-On RPE1 cell line expressing inducible MBP-trichoplein-Flag (MBP-tricho-Flag) was incubated with (+) or without (−) 100 ng/ml Dox in the growing medium for 16 h and then in the serum-free medium for the indicated time. (B and C) Tet-On RPE1 cells were transfected with the indicated siRNA. 6 h after transfection, the cells were incubated with (+) or without (−) Dox for an additional 42 h. (D and E) Proliferating RPE1 cells were treated with combination of the indicated siRNA(s) for 48 h. The graph in D indicates the percentage of ciliated cells; n.s., not significant. The data are shown as mean ± SEM from three independent experiments (n > 100 each). Bar, 500 nm.
Figure 7.

Primary cilia assembly by Ndel1 depletion is reverted by trichoplein overproduction or KCTD17 coknockdown. (A) Tet-On RPE1 cell line expressing inducible MBP-trichoplein-Flag (MBP-tricho-Flag) was incubated with (+) or without (−) 100 ng/ml Dox in the growing medium for 16 h and then in the serum-free medium for the indicated time. (B and C) Tet-On RPE1 cells were transfected with the indicated siRNA. 6 h after transfection, the cells were incubated with (+) or without (−) Dox for an additional 42 h. (D and E) Proliferating RPE1 cells were treated with combination of the indicated siRNA(s) for 48 h. The graph in D indicates the percentage of ciliated cells; n.s., not significant. The data are shown as mean ± SEM from three independent experiments (n > 100 each). Bar, 500 nm.

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