Figure 6. Exogenous Ndel1 expression inhibits primary cilia assembly in serum-starved cells. (A–D) Tet-On RPE1 cell line expressing inducible GFP (A) or Ndel1-Myc (A–D) was incubated with (+) or without (−) 30 ng/ml Dox in the growing medium for 24 h and then in the serum-free medium for the indicated time. Treated cells were subjected to immunostaining (A, C, and D) or immunoblotting (B). In A, the percentage of ciliated cells was quantified as described in the legend of Fig. 1. As judged by the immunostaining data (C), the proportion of cells with a trichoplein-positive mother centriole (MC) was quantified and normalized to 0 h after serum starvation in each group, which was set at 1.0. The data are shown as mean ± SEM from three independent experiments (n > 100 each; A and D). Bar, 1 µm.
Figure 6.

Exogenous Ndel1 expression inhibits primary cilia assembly in serum-starved cells. (A–D) Tet-On RPE1 cell line expressing inducible GFP (A) or Ndel1-Myc (A–D) was incubated with (+) or without (−) 30 ng/ml Dox in the growing medium for 24 h and then in the serum-free medium for the indicated time. Treated cells were subjected to immunostaining (A, C, and D) or immunoblotting (B). In A, the percentage of ciliated cells was quantified as described in the legend of Fig. 1. As judged by the immunostaining data (C), the proportion of cells with a trichoplein-positive mother centriole (MC) was quantified and normalized to 0 h after serum starvation in each group, which was set at 1.0. The data are shown as mean ± SEM from three independent experiments (n > 100 each; A and D). Bar, 1 µm.

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