Ndel1 knockdown causes primary cilium-dependent cell cycle arrest. (A–F) Proliferating RPE1 cells were treated with the combination of the indicated siRNA(s) as reported previously (Inoko et al., 2012). The treated cells were subjected to immunostaining (A, B, and D), immunoblotting (C), or FACS analysis (E and F). Insets show magnified images of indicated centrosomes in lower micrographs (A). The data are shown as mean ± SEM from three independent experiments (n > 100 each in B). BrdU (D) and FACS (E and F) analyses were performed as described previously (Inoko et al., 2012). Data from a single representative sample are shown (D and E). Bars: (A, main images) 10 µm; (A, insets) 1 µm; (D) 50 µm.