Comparison of ciliary phenotypes among Ndel1, NDE1, Lis1, and dynein. RPE1 cells were treated with Ndel1-, Lis1- (A–D), DYNC1H1- (E–G), DYNC1I2- (H–J), or Tctex-1–specific (K–M) siRNA. We evaluated unscheduled ciliation in proliferating cells (A–C, E, F, H, I, K, and L) or deciliation at cell cycle reentry (D, G, J, and M) as described in the legends of Figs. 1 or 2, respectively. The data are shown as mean ± SEM from three independent experiments (n > 100 each). White arrowheads indicate centrosome or primary cilia; these images were magnified in insets. White dashed line indicates cell borders. Asterisk indicates degraded products (E). Bars: (main) 5 µm; (insets) 1 µm. ac-tub., anti–acetylated tubulin.