TF-dependent and stimulus-specific recruitment of INO1, PRM1, and HIS4 to the nuclear periphery. (A) Confocal micrographs of cells having the Lac operator (LacO) array integrated at INO1, expressing GFP-LacI and an mCherry-ER marker (Egecioglu et al., 2014), scored as either peripheral (top) or nucleoplasmic (bottom). Bar, 1 µm. (B) Wild-type or mutant strains having the LacO array inserted at URA3, INO1, PRM1, or HIS4 were imaged by confocal microscopy under uninducing conditions (synthetic complete medium; gray bars), after inositol starvation overnight (black bars), after α-factor stimulation for 15–25 min (orange bars), or after histidine starvation for 45–75 min (cyan blue bars). (C) P-values (Fisher exact test) comparing the peripheral localization of the URA3 locus with INO1, PRM1, or HIS4 in the indicated conditions and strains from B. -ino, inositol starvation; +α-f, α-factor stimulation; -his, histidine starvation. (D) Peripheral localization of INO1, PRM1, and HIS4 in spt20Δ strain. *, P ≤ 0.05 (Fisher exact test) between SDC and inducing condition for specific strain. Mean and SEM from three of more biological replicates (30–60 cells per replicate).