Figure 5. FGDF motif of USP10 or nsP3 31... | Rockefeller University Press

FGDF motif of USP10 or nsP331 is required to block SG formation. (A) U2OS-WT cells stably expressing GFP-tagged USP10-WT, F10A, Δ1–30, and ΔPAM2 (green) were cocultured with U2OS-WT cells (nongreen), treated with 200 µM AS (1, 4, 7, and 10), 1 µM TG (2, 5, 8, and 11), or 20 µM CZ (3, 6, 9, and 12) and stained for SG markers eIF4G (red) and eIF3b (blue). Bar, 25 µm. (B) U2OS-WT cells transiently transfected with GFP-USP101–40WT, GFP-USP101–40-F10A, GFP-nsP331WT, or GFP-nsP331F3A as indicated and treated with 500 µM AS for 1 h, fixed, and stained for eIF4G (red) and eIF3b (blue). Bar, 25 µm.

Figure 5.

FGDF motif of USP10 or nsP3₃₁ is required to block SG formation. (A) U2OS-WT cells stably expressing GFP-tagged USP10-WT, F10A, Δ1–30, and ΔPAM2 (green) were cocultured with U2OS-WT cells (nongreen), treated with 200 µM AS (1, 4, 7, and 10), 1 µM TG (2, 5, 8, and 11), or 20 µM CZ (3, 6, 9, and 12) and stained for SG markers eIF4G (red) and eIF3b (blue). Bar, 25 µm. (B) U2OS-WT cells transiently transfected with GFP-USP10_1–40WT, GFP-USP10_1–40-F10A, GFP-nsP3₃₁WT, or GFP-nsP3₃₁F3A as indicated and treated with 500 µM AS for 1 h, fixed, and stained for eIF4G (red) and eIF3b (blue). Bar, 25 µm.

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