LSR expression during EAE and MCAO. (A) LSR expression in mouse SC at 4 d after onset of symptoms after induction of EAE. Tissue sections of mouse SC were labeled with antibodies against CD45 (magenta), CD31 (green), endogenous IgG (white), and LSR (red). The broken lines demarcate the inflammatory lesion on the right and the noninflamed region on the left, with larger images for LSR staining (top) and merge picture (bottom). LSR is down-regulated in inflamed blood vessels. Bars, 50 µm. (B) Quantification of LSR expression in blood vessels of inflamed and noninflamed regions after EAE induction. LSR expression is down-regulated in inflamed regions. Data represent mean ± SEM (error bars). ***, P < 0.0001. Unpaired t test. (C) Biotin leakage in mouse brain after MCAO. MCAO was performed on mice for 2 h, followed by 24 h of reperfusion. Mice were then anesthetized and given a transcardiac perfusion of PBS/biotin followed by PFA. Tissue sections were stained for CD31 (green) and streptavidin–Alexa Fluor 594 (to label biotin). Whole brain sections were digitally reconstructed to visualize biotin extravasation. The broken line in the center separates the contralateral hemisphere (left) from the ipsilateral side (right). Boxes indicate where pictures were taken for LSR measurement in D. Bar, 0.5 mm. (D) LSR expression in mouse brain 24 h after reperfusion after MCAO. Tissue sections of mouse brains were colabeled for LSR (red) and blood vessels (BSL, green) in the contralateral (top) and ipsilateral (bottom) hemispheres. Bar, 50 µm. (E) Quantification of LSR expression in the contralateral and ipsilateral hemispheres after MCAO. LSR expression is down-regulated in the ipsilateral hemisphere. Data represent mean ± SEM (error bars). *, P < 0.05. Unpaired t test.