The mitochondria of nup116ΔGLFG nup145ΔGLFG cells exhibit morphological and functional defects. (A) Strains were serially diluted onto glucose or glycerol and incubated at 30 or 37°C until grown as shown. (B) Enriched mother (eight divisions) and young cells (zero divisions) were stained with MitoTracker red CMXRos. ImageJ was used to quantify the mean fluorescence intensity of each cell; ≥10 cells per condition were quantified from three independent experiments. *, P < 0.01 when compared with wild-type zero divisions using Tukey’s post-hoc test after a one-way ANOVA. **, P < 0.01 when compared with wild-type eight divisions using Tukey’s post-hoc test. Error bars represent SEM. (C) Representative single cells from B stained using CCFW and MitoTracker red CMXRos. CCFW images show the top and bottom of cells for visualization of most bud scars. DIC, differential interference contrast. Bar, 2.5 µm. (D) Survival curves of nup116ΔGLFG nup145ΔGLFG cells transformed with an empty or GSP1 2µ vector grown at 30°C. *, P < 0.04 when the curves are compared using a log-rank test (n ≥ 25 cells). (E) Survival curves of wild-type cells or those overexpressing GSP1 under a TDH3 promoter grown at 30°C. *, P < 0.05 when compared with wild type using a log-rank test with n ≥ 30 cells. Mean RLSs are listed in parentheses.