Figure 2.
Figure 2. Pex2-GFP and Pex17-GFP localization in WT and mutant cells. Fluorescence microscopy analysis of methanol-grown cells (3 h) expressing Pex2-GFP or Pex17-GFP. Cells were grown in YPD and switched during exponential phase to methanol medium. DIC, differential interference contrast. Bar, 2 µm. (A) Colocalization of Pex2-GFP expressed from the inducible alcohol oxidase (AOX) promoter with the ER marker Sec61-mCherry in WT and mutant cells. (B) Colocalization of Pex17-GFP expressed from the inducible AOX promoter with the ER marker Sec61-mCherry in WT and mutant cells. (C) Localization of Pex2-GFP and Pex17-GFP with Pex3-RFP after 6 h in methanol medium. Each localization experiment was repeated more than five times with similar results.

Pex2-GFP and Pex17-GFP localization in WT and mutant cells. Fluorescence microscopy analysis of methanol-grown cells (3 h) expressing Pex2-GFP or Pex17-GFP. Cells were grown in YPD and switched during exponential phase to methanol medium. DIC, differential interference contrast. Bar, 2 µm. (A) Colocalization of Pex2-GFP expressed from the inducible alcohol oxidase (AOX) promoter with the ER marker Sec61-mCherry in WT and mutant cells. (B) Colocalization of Pex17-GFP expressed from the inducible AOX promoter with the ER marker Sec61-mCherry in WT and mutant cells. (C) Localization of Pex2-GFP and Pex17-GFP with Pex3-RFP after 6 h in methanol medium. Each localization experiment was repeated more than five times with similar results.

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