Structural differences of low- versus high-Delta kinetochores during metaphase. (A) Correlative LM and EM for a pair of sister kinetochores. Mis12-mCherry (red) and CenpA-GFP (green) fluorescence are shown in the left panel, and corresponding EM serial sections are shown in the remaining panels. (B) Slices from EM tomographic reconstructions of Ka and Kb shown in A. The three columns on the left are individual 2-nm-thin slices from the reconstructions of Ka (top) and Kb (bottom). The height of each slice within the tomogram is indicated. Arrows point at the electron-opaque outer plate. Images in the right column are surface renderings of the electron opaque outer layer segmented from the rest of the reconstruction volume. Delta values are indicated for each kinetochore. (C, left) A single EM section containing a different pair of sister kinetochores. (C, middle and right) Surface renderings of EM tomographic reconstructions of each sister kinetochore with Delta values indicated.