Confocal microscopy and SIM of A10 and γ-secretase in 7W cells. (A) A 1-μm optical section of 7W cells stained for A10 (green), PS1 (red), and Hoechst by confocal microscopy (left). Individual channel image for A10 (green, top right) and PS1 (red, bottom right). (B) Orthogonal view of SIM image showing colocalization (yellow) of some A10 (green) and PS1 (red) immunoreactive puncta in 7W cells. (C) Superresolution SIM images of A10 (green) and PS1 (red) immunoreactive puncta in 7W cells. (D) Magnified image of inset in C. (E) 3D-rendered image of D by Imaris processing. Bars: (A) 20 µm; (B) 2 µm; (C) 4 µm; (D and E) 3 µm.