Figure 4. A10 exists with γ-secretase in... | Rockefeller University Press

Figure 4.

$Figure 4. A10 exists with γ-secretase in an HMW complex isolated from normal brain by SEC. (A) WT mouse brain microsomes were fractionated on a Superose 6 column and blotted with antibodies to the indicated proteins. (B) Mouse brain microsome fractions from a Superose 6 column were pooled into HMW or LMW fractions and immunoprecipitated for A10 and then probed for A10 and coIP of NCT and PS1 CTF. The asterisks indicate nonspecific bands. (C) S20 cells were lysed in 0.25% DDM, loaded onto a BN-PAGE, and probed for A10 and γ-secretase. (D) 7W cells transfected with control or A10 siRNA were analyzed as in C. im, immature; m, mature.$

A10 exists with γ-secretase in an HMW complex isolated from normal brain by SEC. (A) WT mouse brain microsomes were fractionated on a Superose 6 column and blotted with antibodies to the indicated proteins. (B) Mouse brain microsome fractions from a Superose 6 column were pooled into HMW or LMW fractions and immunoprecipitated for A10 and then probed for A10 and coIP of NCT and PS1 CTF. The asterisks indicate nonspecific bands. (C) S20 cells were lysed in 0.25% DDM, loaded onto a BN-PAGE, and probed for A10 and γ-secretase. (D) 7W cells transfected with control or A10 siRNA were analyzed as in C. im, immature; m, mature.