Figure 3. The association between A10 and γ-secretase is observed at endogenous expression levels in cells and WT mouse brain. (A) 7W cells were lysed in 1% CHAPSO in the absence or presence of 1,10-phenanthroline, immunoprecipitated for TACE or just with PAA, and then probed for coIP of PS1 CTF. (B) M17D cells were lysed in CHAPSO with 1,10-phenanthroline. Lysates were immunoprecipitated for TACE or A10 and probed for coIP of PS1 CTF. (C) Microsomes prepared from WT mouse brains were immunoprecipitated for A10 with two different antibodies (19026 and 422751) and blotted for the coIP of PS1 CTF. (D and E) Mouse brain microsomes were immunoprecipitated for TACE, A10, or ADAM9 (A9) and controls of PAA only or TFR and probed for coIP of PS1 CTF, TFR, TACE, and A10. The asterisks indicate nonspecific bands. (F) Mouse brain microsomes were immunoprecipitated for PS1 CTF and blotted for coIP of A10, PS1, and APH-1. im, immature; m, mature.
Figure 3.

The association between A10 and γ-secretase is observed at endogenous expression levels in cells and WT mouse brain. (A) 7W cells were lysed in 1% CHAPSO in the absence or presence of 1,10-phenanthroline, immunoprecipitated for TACE or just with PAA, and then probed for coIP of PS1 CTF. (B) M17D cells were lysed in CHAPSO with 1,10-phenanthroline. Lysates were immunoprecipitated for TACE or A10 and probed for coIP of PS1 CTF. (C) Microsomes prepared from WT mouse brains were immunoprecipitated for A10 with two different antibodies (19026 and 422751) and blotted for the coIP of PS1 CTF. (D and E) Mouse brain microsomes were immunoprecipitated for TACE, A10, or ADAM9 (A9) and controls of PAA only or TFR and probed for coIP of PS1 CTF, TFR, TACE, and A10. The asterisks indicate nonspecific bands. (F) Mouse brain microsomes were immunoprecipitated for PS1 CTF and blotted for coIP of A10, PS1, and APH-1. im, immature; m, mature.

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