Monopolar attachment of homologous kinetochores in sentin oocytes. (A) Live stage 13 oocytes expressing GFP-Mis12 and Rcc1-mCherry. (B) Stage 14 oocytes expressing GFP-Mis12 and Rcc1-mCherry. (C) The frequency of asymmetric kinetochore distributions seen by GFP-Mis12 (n = 26, 11, 31, and 29). (D) The frequencies of position change of asymmetrically distributed kinetochores within 20 min, seen by GFP-Mis12 (n = 5, 10, 0, and 12). ND, not done because of no control stage 14 oocytes with asymmetrically distributed kinetochores observable for 20 min. (E) Live mature oocytes expressing GFP-Rod and Rcc1-mCherry. Arrowheads point to accumulated Rod-GFP. kMT, kinetochore microtubule; KC, kinetochore. (F) Diagrams of various attachment modes with expected Rod-GFP localization. Only monopolar attachments can explain the unseparated homologous centromeres observed in sentin-mutant oocytes. (G) The frequency of oocytes with at least one kinetochore accumulating Rod-GFP (n = 25, 20, 10, 12, 18, and 21). *, P < 0.05; **, P < 0.01; ***, P < 0.001, significant differences from the control. Error bars indicate the 95% confidence intervals. Bars, 10 µm.