Figure 4.
Figure 4. Perinuclear localization of NMIIB is not dependent on formation of NMIIA fibers. (A) MDA-MB 231 NT shRNA control or NMIIA shRNA cells were plated on fibronectin-coated coverglass and fixed at 1, 2, or 16 h and stained for NMIIB and pMLC. Arrowheads indicate lateral stress fibers of NMIIB. Dashed boxes indicate region of detail for B. (B) Details of boxes from A. Activated NMIIB fibers in both control and NMIIA shRNA cells were observed below and above the nucleus. All images were acquired with identical parameters, and contrast enhancements were all performed identically. Bars, 10 µm.

Perinuclear localization of NMIIB is not dependent on formation of NMIIA fibers. (A) MDA-MB 231 NT shRNA control or NMIIA shRNA cells were plated on fibronectin-coated coverglass and fixed at 1, 2, or 16 h and stained for NMIIB and pMLC. Arrowheads indicate lateral stress fibers of NMIIB. Dashed boxes indicate region of detail for B. (B) Details of boxes from A. Activated NMIIB fibers in both control and NMIIA shRNA cells were observed below and above the nucleus. All images were acquired with identical parameters, and contrast enhancements were all performed identically. Bars, 10 µm.

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