Figure 3.
Figure 3. RICS analysis of the mobility of various Gag mutants. (A) Schematic representation and RICS analysis of the different point mutants of Gag used in this work. Shown on the right are representative, normalized, experimental SACFs of the mobile fraction of wt Gag and given mutants. Data are color coded according to the value of the goodness-of-fit weighted residuals parameter rw, where gray illustrates a good fit and red–blue indicates regions where the residuals deviate by >2.5 σ. The narrower shape of the SACF for the constructs with mutations in the NC domain indicates higher mobility for these mutants. (B) Column graph with the diffusion coefficients (left) and mobile fraction (right) determined from fits to the SACF. Error bars are the SDs of n experiments (see Table S1 for n). For proteins containing NC mutations, the mobile fraction is statistically larger than in experiments with mutants that do not have the NC mutations.

RICS analysis of the mobility of various Gag mutants. (A) Schematic representation and RICS analysis of the different point mutants of Gag used in this work. Shown on the right are representative, normalized, experimental SACFs of the mobile fraction of wt Gag and given mutants. Data are color coded according to the value of the goodness-of-fit weighted residuals parameter rw, where gray illustrates a good fit and red–blue indicates regions where the residuals deviate by >2.5 σ. The narrower shape of the SACF for the constructs with mutations in the NC domain indicates higher mobility for these mutants. (B) Column graph with the diffusion coefficients (left) and mobile fraction (right) determined from fits to the SACF. Error bars are the SDs of n experiments (see Table S1 for n). For proteins containing NC mutations, the mobile fraction is statistically larger than in experiments with mutants that do not have the NC mutations.

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