AMIGO2 modulates the PDK1–Akt pathway. (A) The endogenous effect of AMIGO2 knockdown on Akt signaling in HUVECs. Two types of siA2 were evaluated. (B) Endogenous effects of AMIGO overexpression on Akt signaling activation in HUVECs. (C and D) Akt phosphorylation in hyaloid vessels (C) and retinas (D) from Amigo2-depleted mice was evaluated. The data are representative of six mice. (E) Temporal effects of AMIGO2 knockdown on VEGF-induced signaling pathways (20 ng/ml) in HUVECs. (F) VEGF-induced PI3K–PDK1–Akt signaling was evaluated in AMIGO2 siRNA–transfected HUVECs.