Figure 2. Microtubule-dependent events... | Rockefeller University Press

$Figure 2. Microtubule-dependent events contribute fractionally to off-axis fluctuations. (A, left) Image of metaphase yeast with pericentric chromatin (6.8 kb from CEN15) labeled in green and SPBs (Spc29-RFP) labeled in red. (middle) Corresponding kymographs of single-plane time-lapses of motion parallel to the spindle axis. (right) Kymograph of motion perpendicular to the spindle axis. Arrows indicate an excursion event. Left SPB is fixed in position. Bar, 0.5 µm. (B, left) Image of metaphase cells treated with low-concentrations of benomyl. (middle) Corresponding kymographs of single-plane time-lapses of motion parallel to the spindle axis. (right) Kymograph showing motion perpendicular to the spindle axis. Left SPB is fixed in position. (C, left) Image of cfb5-AUU mutant cells with a LacO/LacI-GFP array 12.5-kb from CEN11 and labeled SPBs (red). (middle) Kymographs of single-plane time-lapses of motion parallel to the spindle axis. (right) Kymograph of motion perpendicular to the spindle axis. T is the top GFP foci, B is the bottom GFP foci. The bottom GFP foci stretches, giving it an elongated appearance in the kymograph.$

Figure 2.

Microtubule-dependent events contribute fractionally to off-axis fluctuations. (A, left) Image of metaphase yeast with pericentric chromatin (6.8 kb from CEN15) labeled in green and SPBs (Spc29-RFP) labeled in red. (middle) Corresponding kymographs of single-plane time-lapses of motion parallel to the spindle axis. (right) Kymograph of motion perpendicular to the spindle axis. Arrows indicate an excursion event. Left SPB is fixed in position. Bar, 0.5 µm. (B, left) Image of metaphase cells treated with low-concentrations of benomyl. (middle) Corresponding kymographs of single-plane time-lapses of motion parallel to the spindle axis. (right) Kymograph showing motion perpendicular to the spindle axis. Left SPB is fixed in position. (C, left) Image of cfb5-AUU mutant cells with a LacO/LacI-GFP array 12.5-kb from CEN11 and labeled SPBs (red). (middle) Kymographs of single-plane time-lapses of motion parallel to the spindle axis. (right) Kymograph of motion perpendicular to the spindle axis. T is the top GFP foci, B is the bottom GFP foci. The bottom GFP foci stretches, giving it an elongated appearance in the kymograph.

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